FASEB J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
fj.05-5678fjev1
20/9/1540    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jernås, M.
Right arrow Articles by Lönn, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jernås, M.
Right arrow Articles by Lönn, M.
Published online before print June 5, 2006 as doi: 10.1096/fj.05-5678fje.

Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression

Margareta Jernås, Jenny Palming, Kajsa Sjöholm, Eva Jennische, Per-Arne Svensson, Britt G. Gabrielsson, Max Levin, Anders Sjögren, Mats Rudemo, Theodore C. Lystig, Björn Carlsson, Lena M. S. Carlsson, and Malin Lönn

E-mail contact: malin.lonn@medic.gu.se

Enlarged adipocytes are associated with insulin resistance and are an independent predictor of type 2 diabetes. To understand the molecular link between these diseases and adipocyte hypertrophy, we developed a technique to separate human adipocytes from an adipose tissue sample into populations of small cells (mean 57.6±3.54 µm) and large cells (mean 100.1±3.94 µm). Microarray analysis of the cell populations separated from adipose tissue from three subjects identified 14 genes, of which five immune-related, with more than fourfold higher expression in large cells than small cells. Two of these genes were serum amyloid A (SAA) and transmembrane 4 L six family member 1 (TM4SF1). Real-time RT-PCR analysis of SAA and TM4SF1 expression in adipocytes from seven subjects revealed 19-fold and 22-fold higher expression in the large cells, respectively, and a correlation between adipocyte size and both SAA and TM4SF1 expression. The results were verified using immunohistochemistry. In comparison with 17 other human tissues and cell types by microarray, large adipocytes displayed by far the highest SAA and TM4SF1 expression. Thus, we have identified genes with markedly higher expression in large, compared with small, human adipocytes. These genes may link hypertrophic obesity to insulin resistance/type 2 diabetes.--Jernås, M., Palming, J., Sjöholm, K., Jennische, E., Svensson, P.-A., Gabrielsson, B. G., Levin, M., Sjögren, A., Rudemo, M., Lystig, T. C., Carlsson, B., Carlsson, L. M. S., Lönn, M. Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. Beaslas, F. Torreilles, P. Casellas, D. Simon, G. Fabre, M. Lacasa, F. Delers, J. Chambaz, M. Rousset, and V. Carriere
Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes
Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G942 - G952.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Stienstra, C. Duval, S. Keshtkar, J. van der Laak, S. Kersten, and M. Muller
Peroxisome Proliferator-activated Receptor {gamma} Activation Promotes Infiltration of Alternatively Activated Macrophages into Adipose Tissue
J. Biol. Chem., August 15, 2008; 283(33): 22620 - 22627.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
C. H. Chen, P. H. Wang, B. H. Liu, H. H. Hsu, H. J. Mersmann, and S. T. Ding
Serum Amyloid A Protein Regulates the Expression of Porcine Genes Related to Lipid Metabolism
J. Nutr., April 1, 2008; 138(4): 674 - 679.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Gummesson, M. Jernas, P.-A. Svensson, I. Larsson, C. A. M. Glad, E. Schele, L. Gripeteg, K. Sjoholm, T. C. Lystig, L. Sjostrom, et al.
Relations of Adipose Tissue CIDEA Gene Expression to Basal Metabolic Rate, Energy Restriction, and Obesity: Population-Based and Dietary Intervention Studies
J. Clin. Endocrinol. Metab., December 1, 2007; 92(12): 4759 - 4765.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. Maury, K. Ehala-Aleksejev, Y. Guiot, R. Detry, A. Vandenhooft, and S. M. Brichard
Adipokines oversecreted by omental adipose tissue in human obesity
Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E656 - E665.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. Palming, K. Sjoholm, M. Jernas, T. C. Lystig, A. Gummesson, S. Romeo, L. Lonn, M. Lonn, B. Carlsson, and L. M. S. Carlsson
The Expression of NAD(P)H:Quinone Oxidoreductase 1 Is High in Human Adipose Tissue, Reduced by Weight Loss, and Correlates with Adiposity, Insulin Sensitivity, and Markers of Liver Dysfunction
J. Clin. Endocrinol. Metab., June 1, 2007; 92(6): 2346 - 2352.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. R. Coenen, M. L. Gruen, A. Chait, and A. H. Hasty
Diet-Induced Increases in Adiposity, but Not Plasma Lipids, Promote Macrophage Infiltration Into White Adipose Tissue
Diabetes, March 1, 2007; 56(3): 564 - 573.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
T. Skurk, C. Alberti-Huber, C. Herder, and H. Hauner
Relationship between Adipocyte Size and Adipokine Expression and Secretion
J. Clin. Endocrinol. Metab., March 1, 2007; 92(3): 1023 - 1033.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by The Federation of American Societies for Experimental Biology.