| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 8, 939-946, Copyright © 1994 by The Federation of American Societies for Experimental Biology
REVIEWS |
RR Neubig
Department of Pharmacology, University of Michigan, Ann Arbor 48109- 0626.
A prevailing view of receptor and G-protein function in cells includes random collisions between the proteins with a great specificity at the sites of protein-protein interaction. Recent evidence suggests that receptors, G-proteins, and effectors may be less mobile and that these systems are more highly organized than previously appreciated. Several types of evidence suggest that receptors do not have free access to all G-protein with which they are capable of coupling. Also, the specificity of signaling in intact cells appears to be significantly greater than in reconstituted systems. The distribution and mobility of G-proteins in cells are restricted to a surprising degree. Thus, complex interactions of the receptors and G-proteins with their effectors and cell membrane machinery appear to play an important role in their function. A full understanding of G-protein-coupled receptors must include a better description of the organization of these systems in cell membranes. Possible roles for noncoated pits (caveolae) and a novel pleckstrin homology domain need to be examined.
This article has been cited by other articles:
|
|
 |
|
  S. M. Pontier, Y. Percherancier, S. Galandrin, A. Breit, C. Gales, and M. Bouvier Cholesterol-dependent Separation of the {beta}2-Adrenergic Receptor from Its Partners Determines Signaling Efficacy: INSIGHT INTO NANOSCALE ORGANIZATION OF SIGNAL TRANSDUCTION J. Biol. Chem., September 5, 2008; 283(36): 24659 - 24672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Qin, P. R. Sethi, and N. A. Lambert Abundance and stability of complexes containing inactive G protein-coupled receptors and G proteins FASEB J, August 1, 2008; 22(8): 2920 - 2927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ganguly, T. J. Pucadyil, and A. Chattopadhyay Actin Cytoskeleton-Dependent Dynamics of the Human Serotonin1A Receptor Correlates with Receptor Signaling Biophys. J., July 1, 2008; 95(1): 451 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Means, S. Miyamoto, J. Chun, and J. H. Brown S1P1 Receptor Localization Confers Selectivity for Gi-mediated cAMP and Contractile Responses J. Biol. Chem., May 2, 2008; 283(18): 11954 - 11963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Patel, Y. M. Tsutsumi, B. P. Head, I. R. Niesman, M. Jennings, Y. Horikawa, D. Huang, A. L. Moreno, P. M. Patel, P. A. Insel, et al. Mechanisms of cardiac protection from ischemia/reperfusion injury: a role for caveolae and caveolin-1 FASEB J, May 1, 2007; 21(7): 1565 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. V. Iancu, S. W. Jones, and R. D. Harvey Compartmentation of cAMP Signaling in Cardiac Myocytes: A Computational Study Biophys. J., May 1, 2007; 92(9): 3317 - 3331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. El-Yazbi, W. J. Cho, R. Schulz, and E. E. Daniel Caveolin-1 knockout alters beta-adrenoceptors function in mouse small intestine Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1020 - G1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Wells, J. Dingus, and J. D. Hildebrandt Role of the Chaperonin CCT/TRiC Complex in G Protein beta{gamma}-Dimer Assembly J. Biol. Chem., July 21, 2006; 281(29): 20221 - 20232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Franco, V. Casado, J. Mallol, C. Ferrada, S. Ferre, K. Fuxe, A. Cortes, F. Ciruela, C. Lluis, and E. I. Canela The Two-State Dimer Receptor Model: A General Model for Receptor Dimers Mol. Pharmacol., June 1, 2006; 69(6): 1905 - 1912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Gibson and A. G. Gilman Gi{alpha} and G{beta} subunits both define selectivity of G protein activation by {alpha}2-adrenergic receptors PNAS, January 3, 2006; 103(1): 212 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nobles, A. Benians, and A. Tinker Heterotrimeric G proteins precouple with G protein-coupled receptors in living cells PNAS, December 20, 2005; 102(51): 18706 - 18711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Harashima and J. Heitman G{alpha} Subunit Gpa2 Recruits Kelch Repeat Subunits That Inhibit Receptor-G Protein Coupling during cAMP-induced Dimorphic Transitions in Saccharomyces cerevisiae Mol. Biol. Cell, October 1, 2005; 16(10): 4557 - 4571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Maudsley, B. Martin, and L. M. Luttrell The Origins of Diversity and Specificity in G Protein-Coupled Receptor Signaling J. Pharmacol. Exp. Ther., August 1, 2005; 314(2): 485 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Rudajev, J. Novotny, L. Hejnova, G. Milligan, and P. Svoboda Dominant Portion of Thyrotropin-Releasing Hormone Receptor Is Excluded from Lipid Domains. Detergent-Resistant and Detergent-Sensitive Pools of TRH Receptor and Gq{alpha}/G11{alpha} Protein J. Biochem., August 1, 2005; 138(2): 111 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Kerchner, R. L. Clay, G. McCleery, N. Watson, W. E. McIntire, C.-S. Myung, and J. C. Garrison Differential Sensitivity of Phosphatidylinositol 3-Kinase p110{gamma} to Isoforms of G Protein {beta}{gamma} Dimers J. Biol. Chem., October 22, 2004; 279(43): 44554 - 44562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cezanne, S. Lecat, B. Lagane, C. Millot, J.-Y. Vollmer, H. Matthes, J.-L. Galzi, and A. Lopez Dynamic Confinement of NK2 Receptors in the Plasma Membrane: IMPROVED FRAP ANALYSIS AND BIOLOGICAL RELEVANCE J. Biol. Chem., October 22, 2004; 279(43): 45057 - 45067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Hynes, S. M. Mervine, E. A. Yost, J. L. Sabo, and C. H. Berlot Live Cell Imaging of Gs and the {beta}2-Adrenergic Receptor Demonstrates That Both {alpha}s and {beta}1{gamma}7 Internalize upon Stimulation and Exhibit Similar Trafficking Patterns That Differ from That of the {beta}2-Adrenergic Receptor J. Biol. Chem., October 15, 2004; 279(42): 44101 - 44112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Azpiazu and N. Gautam A Fluorescence Resonance Energy Transfer-based Sensor Indicates that Receptor Access to a G Protein Is Unrestricted in a Living Mammalian Cell J. Biol. Chem., June 25, 2004; 279(26): 27709 - 27718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Elenko, T. Fischer, I. Niesman, T. Harding, T. McQuistan, M. Von Zastrow, and M. G. Farquhar Spatial Regulation of G{alpha}i Protein Signaling in Clathrin-Coated Membrane Microdomains Containing GAIP Mol. Pharmacol., July 1, 2003; 64(1): 11 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Molinari, C. Ambrosio, D. Riitano, M. Sbraccia, M. C. Gro, and T. Costa Promiscuous Coupling at Receptor-Galpha Fusion Proteins. THE RECEPTOR OF ONE COVALENT COMPLEX INTERACTS WITH THE alpha -SUBUNIT OF ANOTHER J. Biol. Chem., April 25, 2003; 278(18): 15778 - 15788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhong, S. M. Wade, P. J. Woolf, J. J. Linderman, J. R. Traynor, and R. R. Neubig A Spatial Focusing Model for G Protein Signals. REGULATOR OF G PROTEIN SIGNALING (RGS) PROTEIN-MEDIATED KINETIC SCAFFOLDING J. Biol. Chem., February 21, 2003; 278(9): 7278 - 7284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Daumas, N. Destainville, C. Millot, A. Lopez, D. Dean, and L. Salome Confined Diffusion Without Fences of a G-Protein-Coupled Receptor as Revealed by Single Particle Tracking Biophys. J., January 1, 2003; 84(1): 356 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Christopoulos and T. Kenakin G Protein-Coupled Receptor Allosterism and Complexing Pharmacol. Rev., June 1, 2002; 54(2): 323 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Berlot A Highly Effective Dominant Negative alpha s Construct Containing Mutations That Affect Distinct Functions Inhibits Multiple Gs-coupled Receptor Signaling Pathways J. Biol. Chem., May 31, 2002; 277(23): 21080 - 21085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S Ostrom New Determinants of Receptor-Effector Coupling: Trafficking and Compartmentation in Membrane Microdomains Mol. Pharmacol., March 1, 2002; 61(3): 473 - 476. [Full Text] [PDF]
|
|
|
|
|
|
T. Lockwich, B. B. Singh, X. Liu, and I. S. Ambudkar Stabilization of Cortical Actin Induces Internalization of Transient Receptor Potential 3 (Trp3)-associated Caveolar Ca2+ Signaling Complex and Loss of Ca2+ Influx without Disruption of Trp3-Inositol Trisphosphate Receptor Association J. Biol. Chem., November 2, 2001; 276(45): 42401 - 42408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Alt, I. J. McFadyen, C. D. Fan, J. H. Woods, and J. R. Traynor Stimulation of Guanosine-5'-O-(3-[35S]thio)triphosphate Binding in Digitonin-Permeabilized C6 Rat Glioma Cells: Evidence for an Organized Association of {micro}-Opioid Receptors and G Protein J. Pharmacol. Exp. Ther., July 1, 2001; 298(1): 116 - 121. [Abstract] [Full Text]
|
|
|
|
|
|
R. J. Donati, C. Thukral, and M. M. Rasenick Chronic Treatment of C6 Glioma Cells with Antidepressant Drugs Results in a Redistribution of Gsalpha Mol. Pharmacol., June 1, 2001; 59(6): 1426 - 1432. [Abstract] [Full Text]
|
|
|
|
 |
|
 H.-S. Li, P. A. Hebda, L. A. Kelly, G. D. Ehrlich, D. C. Whitcomb, and J. E. Dohar Up-regulation of Prostaglandin EP4 Receptor Messenger RNA in Fetal Rabbit Skin Wound Arch Otolaryngol Head Neck Surg, November 1, 2000; 126(11): 1337 - 1343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-S. Li and C. Montell TRP and the PDZ Protein, INAD, Form the Core Complex Required for Retention of the Signalplex in Drosophila Photoreceptor Cells J. Cell Biol., September 18, 2000; 150(6): 1411 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Ostrom, S. R. Post, and P. A. Insel Stoichiometry and Compartmentation in G Protein-Coupled Receptor Signaling: Implications for Therapeutic Interventions Involving Gs J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 407 - 412. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Dosil, K. A. Schandel, E. Gupta, D. D. Jenness, and J. B. Konopka The C Terminus of the Saccharomyces cerevisiae alpha -Factor Receptor Contributes to the Formation of Preactivation Complexes with Its Cognate G Protein Mol. Cell. Biol., July 15, 2000; 20(14): 5321 - 5329. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Ostrom, J. D. Violin, S. Coleman, and P. A. Insel Selective Enhancement of beta -Adrenergic Receptor Signaling by Overexpression of Adenylyl Cyclase Type 6: Colocalization of Receptor and Adenylyl Cyclase in Caveolae of Cardiac Myocytes Mol. Pharmacol., May 1, 2000; 57(5): 1075 - 1079. [Abstract] [Full Text]
|
|
|
|
|
|
U. Maier, A. Babich, N. Macrez, D. Leopoldt, P. Gierschik, D. Illenberger, and B. Nurnberg Gbeta 5gamma 2 Is a Highly Selective Activator of Phospholipid-dependent Enzymes J. Biol. Chem., April 28, 2000; 275(18): 13746 - 13754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Short, J. L. Boyer, and R. L. Juliano Integrins Regulate the Linkage between Upstream and Downstream Events in G Protein-coupled Receptor Signaling to Mitogen-activated Protein Kinase J. Biol. Chem., April 21, 2000; 275(17): 12970 - 12977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Wellner-Kienitz, K. Bender, T. Meyer, M. Bunemann, and L. Pott Overexpressed A1 Adenosine Receptors Reduce Activation of Acetylcholine-Sensitive K+ Current by Native Muscarinic M2 Receptors in Rat Atrial Myocytes Circ. Res., March 31, 2000; 86(6): 643 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Li, J. C. Bermak, Z. W. Wang, and Q. Y. Zhou Modulation of Dopamine D2 Receptor Signaling by Actin-Binding Protein (ABP-280) Mol. Pharmacol., March 1, 2000; 57(3): 446 - 452. [Abstract] [Full Text]
|
|
|
|
|
|
D. Vorobiov, A. K. Bera, T. Keren-Raifman, R. Barzilai, and N. Dascal Coupling of the Muscarinic m2 Receptor to G Protein-activated K+ Channels via Galpha z and a Receptor-Galpha z Fusion Protein. FUSION BETWEEN THE RECEPTOR AND Galpha z ELIMINATES CATALYTIC (COLLISION) COUPLING J. Biol. Chem., February 11, 2000; 275(6): 4166 - 4170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Evanko, M. M. Thiyagarajan, and P. B. Wedegaertner Interaction with Gbeta gamma Is Required for Membrane Targeting and Palmitoylation of Galpha s and Galpha q J. Biol. Chem., January 14, 2000; 275(2): 1327 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Brydon, F. Roka, L. Petit, P. de Coppet, M. Tissot, P. Barrett, P. J. Morgan, C. Nanoff, A. D. Strosberg, and R. Jockers Dual Signaling of Human Mel1a Melatonin Receptors via Gi2, Gi3, and Gq/11 Proteins Mol. Endocrinol., December 1, 1999; 13(12): 2025 - 2038. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Oliveira, A.C.M. Paiva, and G. Vriend A low resolution model for the interaction of G proteins with G protein-coupled receptors Protein Eng. Des. Sel., December 1, 1999; 12(12): 1087 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Billington, I. P. Hall, S. J. Mundell, J.-L. Parent, R. A. Panettieri Jr., J. L. Benovic, and R. B. Penn Inflammatory and Contractile Agents Sensitize Specific Adenylyl Cyclase Isoforms in Human Airway Smooth Muscle Am. J. Respir. Cell Mol. Biol., November 1, 1999; 21(5): 597 - 606. [Abstract] [Full Text]
|
|
|
|
|
|
M. Waldhoer, A. Wise, G. Milligan, M. Freissmuth, and C. Nanoff Kinetics of Ternary Complex Formation with Fusion Proteins Composed of the A1-Adenosine Receptor and G Protein alpha -Subunits J. Biol. Chem., October 22, 1999; 274(43): 30571 - 30579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. I. Krieger-Brauer, P. K. Medda, U. Hebling, and H. Kather An Antibody Directed against Residues 100-119 within the alpha -Helical Domain of Galpha s Defines a Novel Contact Site for beta -Adrenergic Receptors J. Biol. Chem., October 1, 1999; 274(40): 28308 - 28313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Seifert, U. Gether, K. Wenzel-Seifert, and B. K. Kobilka Effects of Guanine, Inosine, and Xanthine Nucleotides on beta 2-Adrenergic Receptor/Gs Interactions: Evidence for Multiple Receptor Conformations Mol. Pharmacol., August 1, 1999; 56(2): 348 - 358. [Abstract] [Full Text]
|
|
|
|
|
|
F. D. Smith, G. S. Oxford, and S. L. Milgram Association of the D2 Dopamine Receptor Third Cytoplasmic Loop with Spinophilin, a Protein Phosphatase-1-interacting Protein J. Biol. Chem., July 9, 1999; 274(28): 19894 - 19900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schwencke, M. Yamamoto, S. Okumura, Y. Toya, S.-J. Kim, and Y. Ishikawa Compartmentation of Cyclic Adenosine 3',5'-Monophosphate Signaling in Caveolae Mol. Endocrinol., July 1, 1999; 13(7): 1061 - 1070. [Abstract] [Full Text]
|
|
|
|
 |
|
 S.-W. Jeong and S. R. Ikeda Sequestration of G-Protein beta gamma Subunits by Different G-Protein alpha Subunits Blocks Voltage-Dependent Modulation of Ca2+ Channels in Rat Sympathetic Neurons J. Neurosci., June 15, 1999; 19(12): 4755 - 4761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Edwards and L. E. Limbird Role for the Third Intracellular Loop in Cell Surface Stabilization of the alpha 2A-Adrenergic Receptor J. Biol. Chem., June 4, 1999; 274(23): 16331 - 16336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Prezeau, J. G. Richman, S. W. Edwards, and L. E. Limbird The zeta Isoform of 14-3-3 Proteins Interacts with the Third Intracellular Loop of Different alpha 2-Adrenergic Receptor Subtypes J. Biol. Chem., May 7, 1999; 274(19): 13462 - 13469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Anis, B. Nurnberg, L. Visochek, N. Reiss, Z. Naor, and M. Cohen-Armon Activation of Go-proteins by Membrane Depolarization Traced by in Situ Photoaffinity Labeling of Galpha o-proteins with [alpha 32P]GTP-azidoanilide J. Biol. Chem., March 12, 1999; 274(11): 7431 - 7440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Huang, J. A. Duncan, A. G. Gilman, and S. M. Mumby Persistent membrane association of activated and depalmitoylated G protein alpha subunits PNAS, January 19, 1999; 96(2): 412 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Lindorfer, C.-S. Myung, Y. Savino, H. Yasuda, R. Khazan, and J. C. Garrison Differential Activity of the G Protein beta 5gamma 2 Subunit at Receptors and Effectors J. Biol. Chem., December 18, 1998; 273(51): 34429 - 34436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Blayney, P. Gapper, and C. Rix Identification of phospholipase C {beta} isoforms and their location in cultured vascular smooth muscle cells of pig, human and rat Cardiovasc Res, December 1, 1998; 40(3): 564 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jakubík and T. Haga Effects of an Agonist, Allosteric Modulator, and Antagonist on Guanosine-gamma -[35S]thiotriphosphate Binding to Liposomes with Varying Muscarinic Receptor/Go Protein Stoichiometry Mol. Pharmacol., November 1, 1998; 54(5): 899 - 906. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. W. Shaul and R. G. W. Anderson Role of plasmalemmal caveolae in signal transduction Am J Physiol Lung Cell Mol Physiol, November 1, 1998; 275(5): L843 - L851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Sodickson and B. P. Bean Neurotransmitter Activation of Inwardly Rectifying Potassium Current in Dissociated Hippocampal CA3 Neurons: Interactions among Multiple Receptors J. Neurosci., October 15, 1998; 18(20): 8153 - 8162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Drmota, J. Novotny, G.-D. Kim, K. A. Eidne, G. Milligan, and P. Svoboda Agonist-induced Internalization of the G Protein G11alpha and Thyrotropin-releasing Hormone Receptors Proceed on Different Time Scales J. Biol. Chem., August 21, 1998; 273(34): 21699 - 21707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Haley, F. C. Abogadie, P. Delmas, M. Dayrell, Y. Vallis, G. Milligan, M. P. Caulfield, D. A. Brown, and N. J. Buckley The alpha Subunit of Gq Contributes to Muscarinic Inhibition of the M-Type Potassium Current in Sympathetic Neurons J. Neurosci., June 15, 1998; 18(12): 4521 - 4531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Youn, S. A. Kim, and C.-M. Hai Length-dependent modulation of smooth muscle activation: effects of agonist, cytochalasin, and temperature Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1601 - C1607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Burt, M. Sautel, M. A. Wilson, S. Rees, A. Wise, and G. Milligan Agonist Occupation of an alpha 2A-Adrenoreceptor-Gi1alpha Fusion Protein Results in Activation of Both Receptor-linked and Endogenous Gi Proteins. COMPARISONS OF THEIR CONTRIBUTIONS TO GTPase ACTIVITY AND SIGNAL TRANSDUCTION AND ANALYSIS OF RECEPTOR-G PROTEIN ACTIVATION STOICHIOMETRY J. Biol. Chem., April 24, 1998; 273(17): 10367 - 10375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-J. Chen, S. Armour, J. Way, G. Chen, C. Watson, P. Irving, J. Cobb, S. Kadwell, K. Beaumont, T. Rimele, et al. Expression Cloning and Receptor Pharmacology of Human Calcitonin Receptors from MCF-7 Cells and Their Relationship to Amylin Receptors Mol. Pharmacol., December 1, 1997; 52(6): 1164 - 1175. [Abstract] [Full Text]
|
|
