| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
E-mail contact: m.zhao{at}abdn.ac.uk, c.mccaig{at}abdn.ac.uk
Directed cell migration is essential for tissue formation, inflammation, and wound healing. Chemotaxis plays a major role in these situations and is underpinned by asymmetric intracellular signaling. Endogenous electric fields (EFs) are common where cell movement occurs, such as in wound healing, and cells respond to electric field gradients by reorienting and migrating directionally (galvanotaxis/electrotaxis). We show that a physiological EF redistributed both EGF (epidermal growth factor) receptors and detergent-insoluble membrane lipids asymmetrically, leading to cathodal polarization and enhanced activation of the MAP kinase, ERK1/2. This induced leading-edge actin polymerization in directionally migrating mammalian epithelial cells. Inhibiting the EGF receptor-MAP kinase signaling pathway significantly decreased leading edge actin asymmetry and directional migration. We propose a model in which EF-polarized membrane lipid domains and EGF receptors cause asymmetric signaling through MAP kinase, which drives directional cell migration. A comparison is made with the mechanisms underpinning chemotaxis.
Key words: directional cell migration · electric fields · EGF receptor · MAP kinase ERK1/2 · wound healing · eletrotaxis/galvanotaxis
This article has been cited by other articles:
|
|
 |
|
  C. D. McCaig, B. Song, and A. M. Rajnicek Electrical dimensions in cell science J. Cell Sci., December 1, 2009; 122(23): 4267 - 4276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pu, C. D. McCaig, L. Cao, Z. Zhao, J. E. Segall, and M. Zhao EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells J. Cell Sci., October 1, 2007; 120(19): 3395 - 3403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. V. Gabel, H. Gabel, D. Pavlichin, A. Kao, D. A. Clark, and A. D. T. Samuel Neural Circuits Mediate Electrosensory Behavior in Caenorhabditis elegans J. Neurosci., July 11, 2007; 27(28): 7586 - 7596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Rajnicek, L. E. Foubister, and C. D. McCaig Temporally and spatially coordinated roles for Rho, Rac, Cdc42 and their effectors in growth cone guidance by a physiological electric field J. Cell Sci., May 1, 2006; 119(9): 1723 - 1735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. McCaig, A. M. Rajnicek, B. Song, and M. Zhao Controlling Cell Behavior Electrically: Current Views and Future Potential Physiol Rev, July 1, 2005; 85(3): 943 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Hunton, W. G. Barnes, J. Kim, X.-R. Ren, J. D. Violin, E. Reiter, G. Milligan, D. D. Patel, and R. J. Lefkowitz {beta}-Arrestin 2-Dependent Angiotensin II Type 1A Receptor-Mediated Pathway of Chemotaxis Mol. Pharmacol., April 1, 2005; 67(4): 1229 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pu and M. Zhao Golgi polarization in a strong electric field J. Cell Sci., March 15, 2005; 118(6): 1117 - 1128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Song, M. Zhao, J. Forrester, and C. McCaig Nerve regeneration and wound healing are stimulated and directed by an endogenous electrical field in vivo J. Cell Sci., September 15, 2004; 117(20): 4681 - 4690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Mycielska and M. B. A. Djamgoz Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease J. Cell Sci., May 1, 2004; 117(9): 1631 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mira, R. A. Lacalle, J. M. Buesa, G. G. de Buitrago, S. Jimenez-Baranda, C. Gomez-Mouton, C. Martinez-A, and S. Manes Secreted MMP9 promotes angiogenesis more efficiently than constitutive active MMP9 bound to the tumor cell surface J. Cell Sci., May 1, 2004; 117(9): 1847 - 1857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Shanley, C. D. McCaig, J. V. Forrester, and M. Zhao Insulin, Not Leptin, Promotes In Vitro Cell Migration to Heal Monolayer Wounds in Human Corneal Epithelium Invest. Ophthalmol. Vis. Sci., April 1, 2004; 45(4): 1088 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gomez-Mouton, R. A. Lacalle, E. Mira, S. Jimenez-Baranda, D. F. Barber, A. C. Carrera, C. Martinez-A., and S. Manes Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis J. Cell Biol., March 1, 2004; 164(5): 759 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhao, H. Bai, E. Wang, J. V. Forrester, and C. D. McCaig Electrical stimulation directly induces pre-angiogenic responses in vascular endothelial cells by signaling through VEGF receptors J. Cell Sci., January 22, 2004; 117(3): 397 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Song, M. Zhao, J. V. Forrester, and C. D. McCaig Electrical cues regulate the orientation and frequency of cell division and the rate of wound healing invivo PNAS, October 15, 2002; 99(21): 13577 - 13582. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
