FASEB J. Cell Migration Consortium
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(The FASEB Journal. 2008;22:lb611)
© 2008 FASEB
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Mavria, G.
Right arrow Articles by Marshall, C. J
PubMed
Right arrow Articles by Mavria, G.
Right arrow Articles by Marshall, C. J

lb611

Role of MAP-kinase, Rho GTPases and actomyosin contractility in endothelial cell migration and vessel establishment

Georgia Mavria, Sabu Abraham, Maggie Yeo and Christopher J Marshall

Cell and Molecular Biology, Institute of Cancer Research, London, United Kingdom

ABSTRACT

Cell movement plays a central role in the generation of tumour blood vessels. We are studying how signalling pathways determine movement of endothelial cells. During tube formation studied in an organotypic tissue culture angiogenesis assay, endothelial cells migrate in a 3-dimensional matrix shed by fibroblasts by extending cell protrusions. We have shown that during migration MAPK-ERK opposes Rho-kinase signalling to allow endothelial cell survival and sprouting (1). However, during establishment Rho-kinase activity is up-regulated at cell junctions and is required for the maintenance of vessel quiescence. Inhibition of Rho-kinase switches established tubules to a sprouting phenotype that is Rac dependent. A key determinant of angiogenic sprouting versus establishment is the degree of actomyosin contractility. We show that actomyosin contractility is regulated by Rho family GTPases and VE-cadherin mediated cell-cell adhesion signalling pathways.

REFERENCES





This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Mavria, G.
Right arrow Articles by Marshall, C. J
PubMed
Right arrow Articles by Mavria, G.
Right arrow Articles by Marshall, C. J