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This Article Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Adamko, D. J. Articles by Davoine, F. PubMed Articles by Adamko, D. J. Articles by Davoine, F.

Immunomodulatory potential of CVT-E002 , a proprietary North American Ginseng extract, in an in vitro model of virus-induced asthma exacerbation

¹ Pediatrics,
² Medicine and Faculty St. Jean, University of Alberta, Edmonton, Canada

ABSTRACT

Rationale: Most asthma exacerbations are precipitated by airway virus infections. Understanding the inflammatory response to viruses could allow preventative immune modulating therapies. CVT-E002 (commercially available as COLD fX^®) is a patented poly-furanosyl-pyranosy-saccharide-rich extract of North American ginseng. Clinical trials show that CVT-E002 prevents virus infection. Objectives: To determine whether CVT-E002 has anti-virus properties in vitro; and to understand its effects on human dendritic cells and lymphocytes. Methods: Human dendritic cells (DC) and T cells were cultured with or without virus/virus sham (RSV or RV). Flowcytometry and ELISA assays were performed. Results: RSV and RV upregulate CD25 expression in proliferating memory CD4+ T cells co-cultured with DC (n=3). DC pretreated with CVT-E002 induce increased CD25 on CD4+ T cells similar to virus infection alone. Combination of RV and CVT-E002 augments this expression (n=5). DC with CVT-E002 show increased expression of HLA-DR, CD80, CD86 (n=2). T cells and DC incubated with either RSV or RV release IFN–, IFN-, TNF, and IL-12 (n=1). CVT-E002 pretreatment increases this release. Without virus, CVT-E002 induces some cytokine release. Conclusion: CVT-E002 appears to induce maturation of DC and induces increased T cell proliferation with increased antiviral cytokine responses. CVT-E002 could be a useful immune modulator in the prevention of virus-induced asthma exacerbation.

This Article Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Adamko, D. J. Articles by Davoine, F. PubMed Articles by Adamko, D. J. Articles by Davoine, F.