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(The FASEB Journal. 2008;22:577.1)
© 2008 FASEB
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(The FASEB Journal. 2008;22:577.1-577.)
© 2008 FASEB

577.1

Comparison of placenta-derived MSC (pMSC) with bone marrow MSC (bmMSC) expanded in GMP-compatible medium

Gregor A Pilz1, Julian Braun1, Harald Abele2, Richard Schäfer3 and Wilhelm K. Aicher1

1 Center for Regenerative Medicine, ZMF at UKT, Tübingen, Germany
2 Gynaecology
3 Anestesiology, UKT, Tübingen, Germany

ABSTRACT

Objectives: Human term placenta is a promising source of mesenchymal stromal cells (MSC). After expansion in GMP-compatible medium containing human-platelet-rich plasma, these cells could be utilized in regenerative medicine. In our study we examined the properties of pMSC in comparison to bmMSC.

Methods: PMSC were isolated from term placenta and expanded in FCS-free, GMP-compatible medium. Characteristic MSC antigen expression of plastic adherent cells was observed by flow cytometry. Differentiation to adipogenic, osteogenic and chondrogenic lineages was detected by cytochemistry and immunostaining. Marker gene expression was measured by quantitative RT-PCR.

Results: PMSC can be expanded efficiently in GMP-compatible medium while retaining their typical antigen expression and showing differentiation potential comparable to that of BM MSC.

Conclusion: Human term placenta can be used as a ethically unobjectionable source of MSC and represent a non-invasive alternative to bmMSC. The cultivation of MSC in "GMP-compatible medium" allows rapid expansion to provide the necessary cell numbers for tissue engineering





This Article
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Right arrow Articles by Pilz, G. A
Right arrow Articles by Aicher, W. K.