FASEB J. Cell Migration Consortium
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(The FASEB Journal. 2008;22:148.4)
© 2008 FASEB
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Robbins, R. J.
Right arrow Articles by Gu, L.
PubMed
Right arrow Articles by Robbins, R. J.
Right arrow Articles by Gu, L.
(The FASEB Journal. 2008;22:148.4.)
© 2008 FASEB

148.4

Development of a robust, quantitative normal-phase HPLC method for flavan-3-ols and procyanidin oligomers in chocolate and cocoa-containing samples

Rebecca J. Robbins1, Jadwiga Leonczak1, J. Christopher Johnson1, Julia Li1, Catherine Kwik-Uribe1, Ronald L Prior2 and Liwei Gu3

1 Analytical and Applied Sciences Group, Mars, Incorporated, Hackettstown, NJ
2 Arkansas Children's Nutrition Center, USDA-ARS, Little Rock, AR
3 Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR

ABSTRACT

A novel normal-phase HPLC method for the quantitative determination of flavan-3-ols and procyanidins in chocolate and cocoa-containing food products has been developed. Chromatographic separation based on degree of polymerization (DP = 1–10) was achieved on a diol stationary phase column (normal phase) with a binary gradient of acetonitrile (CH3CN:HOAc) and methanol-water (CH3OH:H2O:HOAc). This novel methodology employs more environmentally friendly solvents than previously published methods and demonstrates greater resolution for larger oligomers. Quantitation was achieved with fluorescent detection (FLD: ex 230 nm/em 321 nm) and the use of external standards. With these solvents, fluorescence quantum yields were significantly greater than observed in previous methods. To have oligomers (DP1 –10) within the detection range of the instrument in a single run, and behave linearly over a wide range of concentrations, an FLD photomultipler tube gain gradient was employed. Sample preparation investigations were conducted to eliminate common chromatographic interferences and assess extraction efficiencies. Method performance was examined on selected cocoa-containing foods (including NIST reference materials) with multiple analysts and laboratories. This method demonstrated accurate and reproducible quantitation of the flavanols and procyanidins in chocolate and cocoa-containing products





This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Robbins, R. J.
Right arrow Articles by Gu, L.
PubMed
Right arrow Articles by Robbins, R. J.
Right arrow Articles by Gu, L.