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Physical performance of myostatin overexpressing transgenic and knock-out mice

¹ Biomedical Sciences, Charles R. Drew University of Medicine and Science, 1731 East 120th Street, Los Angeles, CA, 90059,
² Cerritos High School, 12500 East 183rd St., Cerritos, CA, 90703,
³ El Camino College, 16007 Crenshaw Blvd., Torrance, CA, 90506,
⁴ Division of Respiratory and Critical Care Medicine and Physiology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, 1124 W. Carson St, Torrance, CA, 90502

ABSTRACT

Myostatin (Mst) is one of the transforming growth factors beta (TGF-ß), and is a negative regulator of skeletal muscle mass. It affects muscle function, fiber number and size, myosin heavy chain isoform composition. We aimed at determining the Mst effect on physical performance of adult Mst overexpressing transgenic (TG) and knock out (KO) compared to age-matched wild type (WT) mice (five 1 yr old animals in each group). Skeletal tissue and fat mass were quantified (MicroCAT II CT scan). There was a 24% reduction in hind limb muscle mass and a 1.7 fold increase in abdominal fat in TG animals compared to WT. KO animals had 10% more; TG animals had 22% less fast type fibers than WT. There was no change in fiber number. The spontaneous activity (activity wheel) was the highest (4931±2451 c/day) of the TG animals and KO animals were the least active (2861±944 c/day) (ANOVA N.S.). Training sessions were completed 3 times per week. Each time the treadmill speed was increased by 4 m/min and the inclination by 5% in 5 minute intervals until limit of tolerance. The physical performance after a 6 week training program on treadmill measured as total work (mJ/g) showed that the TG animals had significantly higher exercise capacity (6.6±4.3 mJ/g) compared to KO (1.6±1.6 mJ/g) and WT (2.6±1.2 mJ/g) (SNK P<0.05).

Grants: G12RR030262, S06 GM0685510, P20 MD000545

This Article Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Reisz-Porszasz, S. Articles by Porszasz, J. PubMed Articles by Reisz-Porszasz, S. Articles by Porszasz, J.