(The FASEB Journal. 2007;21:921.31)
© 2007 FASEB
URODYNAMIC CHARACTERIZATION OF MICE LACKING UROPLAKIN II OR III
Tamer Aboushwareb1,
Ge Zho2,
Chanda Turner1,
Karl-Erik Andersson1,
Moses Tar3,
Arnold Melman3,
Fang-Ming Deng2,
Tung-Tien Sun2 and
George J. Christ1
1 Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157,
2 NYU School of Medicine, NYU, New York, NY, 10016,
3 Albert Einstein College of Medicine, AECOM, Bronx, NY, 10461
ABSTRACT
Uroplakins are integral components of urothelial plaques crucial to normal genitourinary function. We examined the effect of gene deletion of uroplakins II (UP2) and III (UP3) on mouse bladder function. Male and female wild type (WT) and knockout (KO) mice lacking uroplakin II (UP2 KO) or uroplakin III (UP3 KO) were evaluated by continuous cystometry in conscious, freely moving animals. Differences in urodynamic parameters (including detrusor overactivity, DO) between genders and between groups were assessed by 2 Way ANOVA. UP2 KO males had larger bladder capacity (BC) than all the other groups. Gender differences between male and female WT mice included: micturition volume (MV) and micturition frequency (MF) (males<females) and bladder compliance (Bcom; males>females). Gender differences in UP2 KO mice included: BC and MV (males>females), basal, threshold, micturition, and intermicturition pressures (males <females), spontaneous activity (SA) and Bcom (males>females), and MF (males<females). UP3KO mice showed no significant gender differences, but UP3 KO females exhibited significantly more SA than WT females. In total, 16/19 UP2 KO (7/9 males; 9/10 females) and 11/19 UP3 KO (5/10 males; 6/9 females) mice exhibited DO, as compared to 3/10 WT animals (1/5 males; 2/5 females). In conclusion, lack of UP, and in particular of UP2, is associated with significant urodynamic changes in bladder function.
