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(The FASEB Journal. 2007;21:873.1)
© 2007 FASEB
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873.1

Heterogeneous signaling pattern of PKC activity associated with the Cav1.2 calcium channel current

E Kobrinsky1, S Thomas1, A Masoudieh1, D A Mager2, D R Abernethy1 and N M Soldatov1

1 National Institute on Aging, Gerontology Research Center, 5600 Nathan Shock Drive, Baltimore, MD, 21224,
2 University of Buffalo at SUNY, Department of Pharmaceutical Sciences, 543 Hochsetter Hall, Buffalo, NY, 14260

ABSTRACT

The role of L-type Cav1.2 voltage gated calcium channels in regulation of protein kinase C (PKC) activity has been studied using FRET-based reporter of PKC and phosphatase activity in COS1 and rat atrial cells under whole-cell patch-clamped conditions. The 2D continuous wavelet transform analysis was applied for detection of signaling microdomains in the plasma membrane. Periodic activation of the calcium current through calcium channels by depolarization pulses to +20 mV for few minutes resulted in appearance of plasma membrane transient microdomains of high phosphatase activity similar to the ones observed on PKC activation with phorbol (Kobrinsky et al., 2005).

After prolong stimulation of calcium channels (10–15 minutes), FRET signal along the plasma membrane became more homogeneous and revealed microdomains of high PKC activity only. Results of our study show that Ca current through the native and recombinant Cav1.2 channels generates a heterogeneous signaling pattern of PKC activity along the plasma membrane.





This Article
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