(The FASEB Journal. 2007;21:552.3)
© 2007 FASEB
Prepartum plane of dietary energy alters hepatic expression of inflammatory and fatty acid oxidation genes in dairy cows
Nicole Anne Janovick-Guretzky,
Heather M. Dann,
Juan J. Loor and
James K. Drackley
Animal Sciences, University of Illinios, 1207 W. Gregory Dr., Urbana, IL, 61801
ABSTRACT
Recent data suggest prepartum plane of energy intake and inflammation may play a role in development of fatty liver and associated disease in dairy cows. Therefore, our objective was to determine transcriptional profiles of mRNA for inflammation-associated genes in the liver. Eighteen multiparous cows were assigned to 1 of 3 prepartum dietary treatments. Diets supplied 80 (80E), 100 (100E) or 150% (150E) of NRC (2001) energy recommendations for non-lactating, late-gestation cows. After parturition, all cows were fed one lactation diet. Total RNA was isolated from liver tissue obtained via biopsy on d -65, -30, -14, 1, 14, and 28 relative to parturition. Quantitative real-time PCR was used to determine expression of mRNA for each gene relative to a control. Genes measured were TNFA, IL1B, SAA3, NFKB, ACOX1, CPT1A, PGC1A, PPARA, SREBF1, PC, NR1H3, and HNF4A. Expression of IL1B was nearly 3 times greater (P < .001) for 150E cows than 80E or 100E cows on d 14. Relative expression of TNFA tended to be greater (P = 0.06) on d -14 for 150E than either 80E or 100E cows. NFKB expression was greater (P = 0.05) and SREBF1 lower (P < 0.001) in 80E cows compared to 150E cows on d -30. Relative expression of ACOX1, CPT1A, PPARA, NR1H3, and HNF4A was greater (P < 0.01) in 100E cows than in 150E cows on d 14. Over-consumption of energy prepartum elicited an inflammatory response in liver and decreased expression of genes related to fatty acid oxidation.
