Supplemental Data

Files in this Data Supplement:

• Supplemental Data - (07-8658SuppData.pdf; 628 KB)

  Figure S1: Abundance of various genes from cDNA library. Pie diagram shows the major groups of genes found in the library and their abundance. Inset: abundance of various novel toxin genes in the library.

  Figure S2: Mutations in the loop regions of β-cardiotoxin. A) Changes in residues between β-cardiotoxin and CTX V₄II from Naja mossambica are highlighted in red over the structure of the CTX V₄II (PDB # 1CDT). B) Changes in residues between -cardiotoxin and other related sequences from O. Hannah venom shown in Fig. 1A. The three loops have been marked using Roman numbers. The image was generated using the DS Viewer Pro software.

  Figure S3 Saturation curves for cloned human β₁- and β₂-ARs produced in SF9 cells. Saturation curve and typical affinity constants for various ligands found with SignalScreen^{registered trademark} cloned human A) β₁-AR and B) β₂-AR. Data provided by PerkinElmer Life and Analytical Sciences (Boston, MA, USA) for the lot used in this study.