Human antimicrobial peptide histatin 5 is a cell-penetrating peptide targeting mitochondrial ATP synthesis in Leishmania FASEB J. Luque-Ortega et al.
22: 1817
Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1
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(07-096081SuppFigure1.tif; 27.9 MB) Dependence of a functional mitochondrion for intracellular accumulation of L-Hst5 or Dhvar4 in L. donovani promastigotes. Parasites preincubated or not with 1 mM KCN, were treated with 10 μM FITC-L-Hst5 (upper row) or 3 μM FITC-Dhvar4 (lower row) according to the standard procedure. The phase contrast patterns (A, C) or fluorescence emission (B, D) were shown.
Supplemental Figure 2
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(07-096081SuppFigure2.ppt; 53 KB) Flow cytometry analysis of DNA content in L donovani promastigotes treated with Hst5. Nucleic acids were stained with PI in order to study the DNA content along the cell cycle for control promastigotes (A), treated with either 15 μM miltefosine (B) or 25 μM L-Hst5 (C). M1 was defined as the region corresponding to sub-G1
peak region, and their respective cell percentages indicated in each histogram.
