The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS FASEB J. Urushitani et al.
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Supplemental Data
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Supplemental Figure 1
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(07-092783SupplFig.tif; 20 MB) EGFP-fused human SOD1 in Golgi vesicles after digitonin treatment. Neuro2a cells were transfected with the plasmids expressing SOD-EGFP and DsRed-Golgi (A and B, WT; C and D, G85R; E and F, G93A). 24 hrs after transfection, cells were treated with 40 μM digitonin for 10 min on ice. Cells were washed and fixed with 4% PFA before fluorescent microscope observation. A, C and E, x 20; B, D and F, x 40 objective lens.
