Tobacco smoke cooperates with interleukin-1ß to alter ß-catenin trafficking in vascular endothelium resulting in increased permeability and induction of cyclooxygenase-2 expression in vitro and in vivo FASEB J. Barbieri and Weksler
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Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1 -
(SupplementalFig1.pdf; 1.08 MB)
Phenotypic characterization of MCEC. (A) MCEC monolayers on gelatin-coated plates. (B) Microtube formation in matrigel MCECs ƒnwere positive for (C) SV40-T (green) and h-TERT (red) nuclear and cytoplasmic staining, respectively, (D) platelet endothelial cell adhesion molecule-1 (PECAM-1), (E) VE-cadherin staining at intercellular junctions, (F) von Willebrand factor-associated antigen (red) in cytoplasm and β-catenin (green) at
intercellular junctions, (G) intense cytoplasmic staining after incubation with 1,1'-dioctadecyl-3,3,3',3;-tetramethyl-indocarbocyanine perchlorate-labeled low-density lipoprotein (Dil-Ac-LDL).
Supplemental Figure 2 -
(SupplementalFig2.pdf; 15 KB)
Time course analyses of cell permeability. MCEC were preincubated in the presence or absence of 1 μmol/L NS398 for 1 hour then stimulated with TS/IL-1β for indicated periods (mean±SD of 4 independent experiments, each performed in duplicate). **P<0.01 vs TS/IL-1β.
Supplemental Figure 3 -
(SupplementalFig3.pdf; 34 KB)
MCEC were stimulated with fresh TS or aged TS (preincubated for 18 hours at 37°C) for the indicated times and processed for Western Blotting.
