LAPSER1 is a putative cytokinetic tumor suppressor that shows the same centrosome and midbody subcellular localization pattern as p80 katanin FASEB J. Sudo and Maru
21: 2086
Supplemental Data
Files in this Data Supplement:
Supplemental Figures -
(06-7254SupplementalData.pdf; 626 KB) Supplemental Figure 1:
(A) Anti-LAPSER1 was pre–incubated with purified GST (GST absorbed-
α-LAPSER1) or GST-LAPSER1-C-terminus (antigenabsorbed-α-LAPSER1). Supplemental Figure 2: (A) Schematic representation of four recombinant LAPSER1 constructs. Supplemental Figure 3: (A) Saos-2 cells were synchronized at the G2/M phase by nocodazole treatment
for 36 hours. Supplemental Figure 4:Saos-2 cells were synchronized at M phase (36 hours after nocodazole release) and subjected to indirect immunofluorescence, as in Supplemental Figure 3. Supplemental Figure 5: (A) Saos-2 cells infected with adeno-LAPSER1-flag virus were immunostained with anti-flag antibodies. Supplemental Figure 6: (A) Lysates from COS7 cells transfected with GFP-LAPSER1, GFP-LAPSER1-ΔLZ, GFP-LAPSER1-ΔN expression constructs were subjected to western blotting with anti-GFP.
