Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - 06-7117SuppFig1.pdf; 509 KB)
  Freshly isolated hepatocytes do not survive photopolymerization in PEG hydrogels but survival and function are improved upon 1 week of cell culture to recover from isolation. Culture conditions were expected to alter integrin expression and were therefore compared in Supplemental Figures 2 and 3. Error bars represent standard deviation of the mean (n=3).
• Supplemental Figure 2 - (06-7117SuppFig2.pdf; 448 KB)
  Relative integrin expression profiles of hepatocytes cultured under various conditions for 1 week as evaluated by flow cytometry. Co-cultivation with stromal cells led to greater mean hepatocyte integrin expression than culture between two layers of collagen I gel (double gel) or culture upon Matrigel. Error bars represent standard deviation of the mean (n=3).
• Supplemental Figure 3 - 06-7117SuppFig3.jpg; 65 KB)
  Alterations in matrix adhesion resulting from changes in integrin expression reported in Supplemental Figure 2. Relative hepatocyte adhesion after 4 h were consistent with flow cytometry data in that co-cultivation led to increased adhesion over other conditions. Bottom panel depicts photomicrographs that indicate qualitative trends that are compiled and quantified by DNA content in top panel. CC indicates co-cultivation with stromal cells, DG indicates double gel, and MG indicates Matrigel. Error bars represent standard deviation of the mean (n=3).
• Supplemental Movie - (06-7117SuppMovie.avi; 16.7 MB)
  3D reconstruction and movie of counter-clockwise 360° image rotation from sequential histological sections of three-layer tissue with fluorescently-labeled cells. Further details on digital volumetric imaging details are described in Materials and Methods.