Functional genomics and biochemical characterization of the C. elegans orthologue of the Machado-Joseph disease protein ataxin-3 FASEB J. Rodrigues et al.
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Supplemental Data
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Supplemental Data
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(06-7002SupplementalData.doc; 3.27 MB) Methods Figure 1: Anti-303JSP specifically recognizes recombinant C. elegans ATX-3. Figure 2:Atx-3 knockout animals are viable and have normal longevity. Figure 3: Atx-3 knockout animals respond normally to heat stress conditions. Figure 4: Chemotaxis and avoidance responses were normal in atx-3 knockout animals. Figure 5: Hierarchical clustering of ratios of the 290 differentially expressed genes at a false discovery rate of 0.1%. Table 1: List of the primers used for nested PCR and cloning. Table 2: List of the primers used for semiquantitative PCR and Real-Time PCR analysis of gene expression.