Telomere 3' overhang-specific DNA oligonucleotides induce autophagy in malignant glioma cells FASEB J. Aoki et al.
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Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1
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(07-6941SupplementalFigure1.ppt; 1.57 MB) Reverse-phase protein microarray assay. Proteins were harvested from U373-MG and U87-MG cells treated with 40 μM C-oligos or T-oligos for 0, 15, 30, or 60 minutes for the assay, and two-fold dilutions up to 1/128 were performed (left). The phosphorylation levels of Akt at Thr308
, Akt at Ser473, mTOR at Ser2448, p38 at Thr180 /Tyr182, STAT3 at Ser727, GSK3 at Ser21/9, and Src at Thy416 were standardized by the equivalent total protein level, and each value was expressed as an intensity showing the ratio of the expression in tumor cells treated with C-oligos or T-oligos to that in untreated cells (right).