Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1 FASEB J. Zhang et al.
21: 3042
Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1
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(06-6914SupplementalFigure1.doc; 190 KB) Endogenous Oct-4 is sumoylated. Lysate from F9 cell stably transfected with Flag-SUMO-1 or empty vector was immunoprecipited with M2 beads and the precipitated proteins were analyzed with anti-Oct-4 antibody.
Supplemental Figure 2
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(06-6914SupplementalFigure2.doc; 212 KB) Sumoylation assays in HEK 293 cells were carried out with wild-type or mutant Oct-4 (K118R or K244R) and C-myc-tagged SUMO-1.