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• PARK ET AL SUPPLEMENTAL FIGURES AND TABLES - SUPPLEMENTAL FIGURE 1 A–F: Differential roles of the proneural bHLH Mash1 and Ngns in Nurr1-induced TH expression. Cortical precursors were transduced with Nurr1 in combination with Mash1, Ngn1, Ngn2 or LacZ (control) at day 2 of expansion. After 2 days of differentiation cultures were subjected to immunocytochemical (A–E) and immunoblot analyses (F) for TH and TuJ1. Representative TH/TuJ1 images are shown in A–D (insets, DAPI nuclear staining of the same field). Graph E represents the percentage of TH+/DAPI+ cells. Increased TuJ1 levels in Ngn1, 2, or Mash1 transduced cells, and a dramatic decrease in TH expression in Ngn1 and Ngn2 but not Mash1 transduced cells are shown in Western-blot analysis (F). G–H, Representative images (G) and morphometric analysis (H) of TH+ cells derived from Nurr1+Mash1 and Nurr1+LacZ cultures at differentiation day 10. Each value was obtained from 25–30 microscopic fields in 3 coverslips. Scale bar, 10ìm. *Statistically significant at p<0.001. SUPPLEMENTAL FIGURE 2 Confocal images of TH+/GFP+ cells in the grafts derived from NHB- or NM-transduced precursor cells. Cultured cortical precursor cells were transduced with mixtures of the individual viral constructs pNurr1-IRES-GFP and pSHH-IRES-Bcl-XL(NHB, 1:1, v:v) or pNurr1-IRES-GFP and pMash1-IRES-LacZ (NM). Eight weeks after transplantation, TH and GFP double immunohistochemistry was performed in the striatal sections of NHB (A–C) or NM (D–F)- grafted animals. Shown in C and F are the merged images of TH (A, D) and GFP (B, E). Scale bar, 20 ìm. SUPPLEMENTAL TABLE 1 Number of animals with >60% recovery in amphetamine-rotation (%) SUPPLEMENTAL TABLE 2 Number of animals with >60% recovery in stepping behavior (%)