Supplemental Data -
(SupplementalData.pdf; 2.24 MB)
Supplemental Figure 1: Northern blot analysis of let-7 expression in mouse brain development.
Supplemental Figure 2: A let-7 sensor construct is differentially regulated in astrocytes and neurons.
Supplemental Figure 3: Representative flow cytometry plots of eGFP expression (GFP Fl-1) versus side scatter (SSC). Gates were established with untransfected cells, for each population the percentage of positive-gated cells is given.
Supplemental Figure 4: Panel A and B: Semi-quantitative RT-PCR analysis of miRNP components. cDNA samples were prepared from mouse embryonic stem cells (ES), P19 embryocarcinoma cells (EC), and primary (Pri.) embryonic astrocytes (A) and neurons (N).
Supplemental Figure 5: Panel A: Western blot comparing Ago1 expression before (U, Lane 1) and after differentiation (N, Lane 2) of EC cells. The position of Ago1 is marked by an arrow to the right.
Supplemental Figure 6: Localization of miRNA processing proteins in cultured hippocampal neurons.
Supplemental Methods
