Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - (SupplFigure1.jpg; 178 KB) General disturbance of calcium physiology in the MCK-βAPP model. (A). Comparisons in membrane potential and (B). resting Ca²⁺_i. (C). Plot of individual fibers. As in Figure 3G, a Non-Tg normal fiber upper cutoff for V_m is 7ndash;80 mV. (D) Graph showing changes in resting Ca²⁺_i. The two populations of Tg fibers observed in the MLC-βAPP mice, were not observed in this mouse model as expected. n = 3 Tg and 3 Non-Tg animals. * Indicates significantly different from control (P<0.05), independent t-test, mean ± SEM.
• Supplemental Figure 2 - (SupplFigure2.jpg; 1.12 MB) MLC1/3-βAPP mice: changes in skeletal muscle tau (A) and (B). Sections of hamstring from animal no.18 (Non-Tg) and animal no.27 (Tg), respectively, showing an increase in immunoreactivity to tau-5 antibody (myoplasmic threads, arrow) in the transgenic (B) compared to non-transgenic control littermate (A). Portions of neurovascular bundles are shown to stain darkly with anti-tau (C) and (D). Scattered intramyofiber immunoreactivity to the MC1 epitope in hamstring of transgenic no.27 (C, arrow) and phase contrast possibly showing inclusion formation (D, arrow). (E). Western blot demonstrating an increase in the level of insoluble mouse tau in the transgenic (no. 15 and 28), compared to non-transgenic (14 and 18) littermates. Samples were fractionated alongside a normal human brain extract (control) on 10% SDS PAGE. Insoluble tau was isolated from muscle specimens that were homogenized in lysis buffer (10 mM Tris, 140 mM NaCl, pH 7.4, 10 mM sodium fluoride, 2 mM EGTA, 1 mM sodium Vanadate, 5% (v/v) &beta-mercaptoethanol, 0.25 mol/L sucrose and 1 mM PMSF) and centrifuged at 100,000g at 4°C for 1h. The insoluble pellet was re-suspended in lysis buffer containing 2% SDS and centrifuged at 10,000g, 4°C for 15 min. The supernatant containing the detergent extractable fraction was collected and analyzed by western blot.