Supplemental Data
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(SupplementalData.doc; 181 KB)Supplemental Methods: Primers used for amplification of DMD inserts
Supplemental Figure 1: Nucleotide sequences spanning the three deletion junctions described in this study (B4-6 in fig. 1) are listed, together with the corresponding normal intron sequences. Upper case and lower case are used to indicate the deletion breakpoints. Characters in bold indicate extra nucleotides at deletion junctions, while underlined characters show microhomologies.
Supplemental Figure 2: Secondary-structure prediction and sodium bisulphite reactivity in the IVS47 region (left panels) and in IVS 71 (right panels). The location of DMD fragments inserted in yeast, as well as of deletion breakpoints, is shown in (A). Light gray identifies the DMD 47-3 insert while the darker gray shading corresponds to the minimal region for DSB formation (insert 47–4). Secondary structure prediction (using the Lempel-Ziv complexity measure) is shown (B) for complementary repeats (blue), symmetric repeats (green), direct repeats (red), and inverted repeats (black). The locations of converted cytosines are shown in panels (C) for the top (blue) and bottom (red) DNA strands as vertical bars. Bar length corresponds to the fraction of molecules that displayed C>T conversion at each position. In the two bottom panels (D), the location of consecutive converted cytosines (more than 7 C) is represented for the IVS47 region (left) and IVS71 (right).
