Nitric oxide regulates mitochondrial oxidative stress protection via the transcriptional coactivator PGC-1

Supplemental Data

Files in this Data Supplement:

• Supplemental Methods and Legends - (SuppData& Legends.pdf; 389 KB)

  Methods:
  Primer pairs used in qRT-PCR
  MGB TaqMan® probes used in qRT-PCR
  Nitric oxide
  Mitochondrial activity
  References

• Supplemental Figures - (SuppFig.ppt.pdf; 607 KB)

  Figure 1: Determination of NO levels and mitochondrial activity
  Figure 2: The effect of NO does not depend on the source of NO
  Figure 3: NO downregulates PGC-1α mRNA expression in HUVEC through the sGC pathway
  Figure 4: Effect of ODQ and KT5823 treatment on PGC-1α mRNA expression levels in BAEC
  Figure 5: cGMP but not cAMP mimicks the negative effect of NO donors on PGC-1α expression
  Figure 6: The effect of NO on the mRNA expression of mitochondrial and oxidative stress protective genes is directly mediated by PGC-1α
  Figure 7: PGC-1α induces eNOS expression in HUVEC