Supplemental Data

Files in this Data Supplement:

• Supplemental Data - (SuppData.pdf; 10.4 MB)

  Supplemental Material 1: Reagents and antibodies.
  Supplemental Method 1: Production of recombinant FSH mutant­occludin peptide (ΔFSH­ocln) conjugate.
  Supplemental Figure 1: A study by electron microscopy to examine the damaging effects of theΔFSH­ ocln conjugate on the BTB in the adult rat testis.
  Supplemental Figure 2: Effects of the administration of ΔFSH­ocln conjugate via i.p. on the localization of occludin at the epithelia of different organs (A–O) and morphology (P–X, and Y) in adult rats.
  Supplemental Table 1: Amino acid sequences of the and subunits of the wild type and mutant FSH proteins.
  Supplemental Table 2: Primers used to produce the recombinant rat FSH mutant (FSH) protein containing two mutation sites plus a 22­amino occludin peptide at the 3′­end in the subunit, and two deletion sites in the subunit.