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Figure 7. Lovastatin treatment enhances the release of neurotrophic factors in the spinal cord of treated-EAE animals and in mixed Glial cell cultures. The mRNA expression of neurotrophic factors was determined by real-time PCR analysis in the spinal cord of LOV-treated/untreated EAE animals and controls as well as CM-treated mixed glial cell cultures as described under Materials and Methods. Graphs represent the mRNA expression of BDNF (A), CNTF (B), PDGF(C), GGF-2 (D), and LIF (E) in the spinal cord of animals from each group. Likewise, graphs represent the mRNA expression for BDNF (F), LIF (G), and GGF-2 (H) in LOV- and CM-treated mixed glial cells. The data in graphs are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and NS (nonsignificant) vs. control, #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. EAE, and $$$P < 0.001 vs. CM.
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