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Figure 2. Reduction of CPD in UVR-irradiated human melanocytes treated with 3.
Melanocytes were plated onto 100 mm dishes, treated with 0 or 1 nM 3 for 4 d, followed by irradiation with 21 mJ/cm2 UVR. DNA was extracted immediately (T0) to measure induction of CPD, or 12 h after UVR exposure (T12) to determine CPD repair, using Southwestern blot analysis. Triplicate lanes were loaded for each group, and the mean ± SE of three different densitometry measurements are presented in the graph. The effect of 3 at T0 and T12 on the UVR-irradiated group was statistically different from the effect of UVR alone at P < 0.05, as determined by ANOVA, followed by Newman-Kewls Multiple Comparison Test. This experiment was repeated three times using two different melanocyte cultures with similar findings.
