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Figure 1. MALDI-TOF MS analysis of MG-induced mass changes of the B-chain of human insulin. A) In the absence of MG, the predominant peak is that of the reduced and alkylated B-chain, with small additional peaks corresponding to attachment of sodium (+22 Da) and a third carbamidomethyl (+57 Da) group. Incubation with increasing concentrations of MG (10, 15, and 30 µM) results in the progressive formation of a dehydrated (+54 Da) MG adduct at m/z 3597, along with other putative B-chain derivatives (B–D).
