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Figure 1. Separation of human adipocytes by size. A) Representative images of human adipocytes from one adipose tissue sample before separation (All) and after separation (Small and Large). Microspheres (98.00 µm in diameter) were used for reference in the computerized image analysis. B) Size distributions, determined by computerized image analysis, and mean diameters in random samples of all adipocytes (n=742), small adipocytes (n=1965), and large adipocytes (n=2382) from one adipose tissue sample. Patient characteristics; man, age: 41 yr, body mass index (BMI): 26.0 kg/m2. C) Mean diameter of small and large adipocytes from 12 adipose tissue biopsies. Error bars indicate SEM.