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Figure 2. CD109 inhibits TGF-ß1-induced responses in human keratinocytes. A) CD109 inhibits TGF-ß1-induced transcriptional activity. HaCaT cells transfected with CD109 or EV were co-transfected with the 3TP-lux reporter construct and ß-galactosidase and treated without or with 100 pM TGF-ß1 for 18 h. Cell lysates were analyzed for luciferase activity and expressed as fold-stimulation ± SD (normalized to ß-galactosidase) relative to activity of cells not treated with TGF-ß1. *P < 0.05. B) CD109 inhibits TGF-ß1-induced PAI-1 and fibronectin protein expression. HaCaT cells tranfected with CD109 or EV were treated without or with 5 or 50 pM TGF-ß1 for 24 h. Cell lysates were analyzed by Western blotting using anti-PAI-1 (top panel), anti-fibronectin (middle panel), or anti-actin (bottom panel) antibodies. The results shown (A, B) are representative of three independent experiments.
