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Figure 1. Effect of promoter differences on eGFP expression in blood cells in vivo. Cultured KSL cells were transduced with SIV-CMV-eGFP or SIV-GPIb
-eGFP at a MOI of 30. Each irradiated mouse received 100,000 transduced cells together with 5 x 105 unfractionated whole marrow cells. A) Representative flow cytometry analyses of eGFP-positive cells in CD45+ lymphocytes and granulocytes, red blood cells (RBCs), and platelets in peripheral blood are shown. B) Percentages of eGFP-positive cells in CD45+ cells (left) and platelets (right) 14, 30, and 60 days after transplantation are shown. Columns and error bars are mean ± SD (n=5 per group). C) 60 days post-transplantation, bone marrow cells were stained using antibodies to detect B lymphocytes (B220), T lymphocytes (CD3), granulocytes (Gr1), macrophages (CD11b), and erythroblasts (TER119). GFP-positive cells in each lineage cells are measured by flow cytometry. Data represent 3 experiments. D) Flow cytometric analyses of CD45+ cells, RBCs, and platelets in peripheral blood obtained from mice 30 days after second bone marrow transplantation. Columns and error bars are mean ± SD (n=5 per group).
