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Figure 2. Telomerase activity and Bmi-1 expression. Telomeric repeat amplification protocol (TRAP; A) and TRF (B) assays were performed on cells transduced with empty vector (V lanes) and antisense p16INK4a-transduced cells (AS-Exo1a lanes) at different cell passages. C) Reporter telomerase promoter, p16INK4a, and lacZ control plasmids were cotransfected in primary keratinocytes. Promoter activities were calculated using the empty vector control (column 1) as reference. The ratio promoter:p16INK4a was 1:1 (column 2), 1:2 (column 3), and 1:3 (column 4). D) c-Myc, Mad1, and Bmi-1 expression was assessed by Western blot using cell extracts from empty vector (V-lanes) and Exo1a (AS-Exo1a-lanes) transduced cells at different cell passages. In A, B, and D, different cell passages were indicated by numbers of cell generations after infection.
