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Figure 1. C-terminal three amino acids (SEA-COOH) of NRP-1 are key functional domains for NRP-1 signaling. A) C-terminal three amino acids (SEA-COOH) of NRP-1 are required for NRP mediated angiogenesis. Confocal images of 2 days and dpf TG(fli1:egfp) embryos. Dorsal is up and anterior is left. Upper three ranks reveal the phenotypes of morpholinos and mRNA treatment. Arrow indicates DLAV; brackets indicate ISV and SIV, respectively; and double arrowheads indicate DA and PCV. Lower rank shows immunoblotting result that reveals protein expression concentration of human hNRP-1
SEA or hNRP-1 in zebrafish. Lower panel) Percentage of vessel defect (n=60, 30, 85, and 120, at 0.1 ng hNRP-1SEA, 0.3 ng hNRP-1SEA, 0.1 ng hNRP-1, and 0.3 ng hNRP-1 injection groups, respectively).
