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Figure 1. Polyclonal antimaspin Ab, but not an anti-RSL Ab generated against the RSL domain of maspin, inhibits MCF-10A cell adhesion to the self-deposited matrix. A) MCF-10A cells were plated on coverslips and stained with the rabbit anti-RSL (AbS4A) Ab on ice, to prevent the internalization of the Ab. Preimmune serum revealed no staining (data not shown). This imagine is representative of two independent assays. Confocal microscope analysis revealed maspin on the cell surface. Bar, 20 µm. B) MCF-10A cells were harvested with enzyme-free dissociation buffer, and preincubated with the indicated antibodies. 2 x 104 cells/well were seeded and cell adhesion was measured after 30 min using colorimetric reaction. Polyclonal anti-maspin inhibited cell adhesion by 76%, whereas no effect was detected with the anti-RSL Ab. Control, preimmune serum. Each sample was measured in triplicate. Result is representative of 3 independent assays. Statistical analysis was done by a t test (P <0.05%).
