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Figure 1. Effect of IL-10, IL-4, and IL-13 on p47PHOX phosphorylation. A) 32Pi-labeled PMN were incubated with GM-CSF (12.5 ng/ml) in the presence or absence of IL-10 (30 ng/ml), IL-4 (30 ng/ml), or IL-13 (30 ng/ml). P47PHOX was detected by autoradiography (P-p47PHOX) or with antip47PHOX Ab (p47PHOX). B, PMN were incubated with GM-CSF (12 ng/ml) in the presence or absence of various concentrations of IL-10. P47PHOX was then detected by autoradiography (P-p47PHOX) or with antip47PHOX Ab (p47PHOX). C) PMN were incubated with GM-CSF (12 ng/ml) or TNF
(4.5 ng/ml) in the presence or absence of IL-10 (30 ng/ml) at 37°C for 20 min. P47PHOX was then detected by autoradiography (P-p47PHOX) or with antip47PHOX Ab (p47PHOX).
