| Yeast iso-1-cytochrome c variant |
Catalytic stability half-life (min) |
Soret band half-life (min) |
kcat (seg–1) |
KM H2O2 (M) |
Catalytic efficiency (kcat/KM) |
|
| WT 16 (T-5A, C102T) |
8.3 |
17.2 |
11.4 |
6.97 x 10–2 |
163 |
| Y46F, Y48F, Y67F, Y74F, Y97F |
0.7 |
45.5 |
8.7 |
5.50 x 10–3 |
1,592 |
| N52I, Y67F |
0.5 |
Stable |
0.01 |
2.76 x 10–3 |
6 |
| N52I, Y67F, M80A |
12.1 |
40.0 |
0.19 |
3.91 x 10–2 |
5 |
| N52I, W59F, Y67F |
0.4 |
Stable |
8.6 |
8.14 x 10–3 |
1,062 |
| N52I, W59F, Y67F, F82G |
91.3 |
Stable |
8.4 |
4.12 x 10–2 |
205 |
| N52I, W59F, Y67F, K79A, F82G |
Stable |
170 |
10.1 |
7.26 x 10–2 |
139 |
|
