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Figure 2. Igf1rb plays a greater role in regulating spontaneous muscle contractility and motoneuron differentiation. A) Percent of embryos exhibiting spontaneous muscle contractility at 24 (filled bar) or 48 hpf (open bar) in the control, igf1ra, or igf1rb MO-injected group. Results were obtained from 3 independent microinjection experiments, with 45 embryos per group. *P < 0.0001. B) Expression pattern of myoD mRNA in 24 hpf control, igf1ra, or igf1rb MO-injected embryos (upper panel). Scale bar = 250 µm. Slow and fast muscle muscles (labeled by F59 staining, red) in 48 hpf control, igf1ra, or igf1rb MO-injected embryos (middle panel). The sections were counterstained with sytox (green, nuclear staining). Scale bar = 20 µm. CaP motoneuron axons (labeled by SV2 staining, dark) in 48 hpf control, igf1ra, and igf1rb MO-injected embryos (bottom panel). Scale bar = 100 µm. Lateral views are shown with anterior to the left and dorsal up.
