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Figure 2. A) Measurement of ·OH and lipid peroxidation in presence or absence of scavengers during malarial infection. Mice (20–25 g) were injected i.p. with different ·OH specific scavengers such as DMSO (500 mg/kg bw), mannitol (500 mg/kg bw), and spin trap PBN (300 mg/kg bw) 1 h before P. yoelii infection. Parasitemia was monitored everyday, and all above-mentioned scavengers and spin trap were administrated to the respective animals daily at doses previously mentioned. On day 4 of infection, when parasitemia was 50–60%, all animals (control, infected, and infected but treated with scavengers) were killed and liver was excised and proceeded for measuring ·OH generation and lipid peroxidation. Data are mean ± SE. (n=6; ***P<0.001 vs. control, ###P<0.001, ## P<0.01 vs. infected). B) Measurement of caspase-3 activity in presence or absence of DMSO, mannitol, and PBN. Mice (20–25 g) were injected (ip) with different ·OH-specific scavengers. Liver was excised and processed for measuring caspase-3 activity. Data are mean ± SE (n=6; ***P<0.001 vs. control, ###P<0.001 vs. infection). C) RT-PCR/densitometric analysis of Bcl-2 and Bax expression (percent relative to control, where control was considered as 100% expression) in liver in presence or absence of scavengers and spin trap. Data are mean ± SE (n=6; ***P<0.001 vs. control, ### P<0.001, ##P<0.01 vs. infected). D) Measurement of 
m in presence or absence of DMSO, mannitol, and PBN. Details of the treatment of ·OH scavengers and PBN were described above. Data are mean ± SE (n=6; ***P<0.001 vs. control, ###P<0.001 vs. infected).
