Table 1. Post-translational modifications of Cx26 and Cx32
| A. |
|
|
|
|
|
|
|
| Source |
m/z |
Calc m/z |
 |
Error (ppm) |
Sequence |
Domain |
Modification |
|
| Mouse |
1650.816 |
1634.821 |
15.99 |
-0.28 |
MDWGTLQSILGGVNK (MSO: 1) |
1–15 NT |
HYDR |
| HeLa |
1714.810 |
1634.821 |
79.97 |
-13.45 |
MDWGTLQSILGGVNK (MSO: 1) |
1–15 NT |
PHOS |
| Mouse |
1308.656 |
1264.642 |
43.99 |
-18.54 |
NEFKDIEEIK |
113–122 CL |
GGLU |
|
| B. |
|
|
|
|
|
|
|
| Source |
m/z |
Calc m/z |
|
Error (ppm) |
Sequence |
Domain |
Modification |
|
| Mouse |
1820.873 |
1740.874 |
79.97 |
-18.1 |
MNWTGLYTLLSGVNR (MSO: 1) |
1–15 NT |
PHOS |
| HeLa |
1756.867 |
1740.874 |
15.99 |
0.93 |
MNWTGLYTLLSGVNR (MSO: 1) |
1–15 NT |
HYDR |
| HeLa |
1820.865 |
1740.874 |
79.97 |
-13.71 |
MNWTGLYTLLSGVNR (MSO: 1) |
1–15 NT |
PHOS |
| Mouse |
1772.883 |
1728.903 |
43.99 |
5.37 |
LEGHGDPLHLEEVKR |
108–122 CL |
GGLU |
| HeLa |
1772.867 |
1728.903 |
43.99 |
14.40 |
LEGHGDPLHLEEVKR |
108–122 CL |
GGLU |
| HeLa |
925.397 |
845.438 |
79.97 |
7.48 |
KGSGFGHR |
231–238 CT |
PHOS |
| HeLa |
1514.668 |
1434.712 |
79.97 |
7.06 |
GSGFGHRLSPEYK |
232–244 CT |
PHOS |
| Mouse |
1036.524 |
798.287 |
238.23 |
-7.16 |
SDRCSAC (1xCys_CAM) |
277–283 CT |
PALM |
|
Differences in post-translational modification of mouse and HeLa Cx26 (A) and Cx32 (B) cytoplasmic domains are highlighted. Residues likely carrying the modifications are bolded and underlined. All modified peptides were also found unmodified in the same digest. See Text and Supplemental Data for further details. Modifications: GGLU, 
-carboxyglutamate; HYDR, hydroxylation; PALM, palmitoylation; PHOS, phosphorylation.
