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Figure 1. A) X-ray spectra collected by SRXRF as described in Materials and Methods. Relative levels of copper and zinc in senile plaques and neuropil are shown in B and C from the sucrose- and nicotine-treated groups, respectively. D) Tested area for scan measurement, a subfield of hippocampal CA1 region. Density maps of copper and zinc in detected area from sucrose-treated group and nicotine-treated group are shown in E. Arrows represent pyramidal neuron layer. Range of mapping is (blue-red) 0–40 for Cu and 0–160 for Zn. Scale bar = 100 µm. $P < 0.05 [plaque (sucrose) vs. neuropil (sucrose)]; #P < 0.05 [plaque (nicotine) vs. neuropil (nicotine)]; *P < 0.05 [plaque (nicotine) vs. plaque (sucrose)]; **P < 0.05 [neuropil (nicotine) vs. neuropil (sucrose)]. Results are mean ± SE, n = 4. Statistical analysis was done by ANOVA.
