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Figure 3. Effects of AAV-INH-2 gene transfer on cardiac function and survival time course in CM hamsters. A) Representative Bluo-gal staining is shown for the LV cross section at 12 wk after AAV-LacZ gene transfer. B) A representative immunoblot is shown for INH-2 at 12 wk after AAV-LacZ gene transfer. C) The time course of %FS (%fibrous sheath) assessed by echocardiography is shown for the LacZ group (open circles) and INH-2 group (solid squares) gene transfer by AAV. *P < 0.05 compared with the LacZ group. D) Microsomal PP1 activity is shown after AAV-mediated gene transfer into the hearts of the LacZ (n=5) and INH-2 groups (n=7). *P < 0.05 compared with the LacZ-treated group. E) Representative immunoblottings are shown for Ser-16 PLN, Thr17 PLN, and total PLN in the LacZ-treated (n=4) and INH-2-treated (n=4) groups. F) Representative images are shown from tissue sections with Azan Mallory staining from the LacZ-treated (n=6) and INH-2-treated (n=7) CM hamsters. Quantification of the percent areas of fibrosis are shown (bottom). *P < 0.05 compared with the LacZ-treated group. G) The Kaplan-Meier plot is shown for the survival of CM hamsters after AAV-mediated gene transfer of LacZ (n=17) and INH-2 (n=13) (P = 0.03).
