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Figure 1. SDS-Polyacrylamide gel electrophoresis of samples obtained during the purification of riproximin. Gels were run with MOPS buffer followed by silver staining. a) Riproximin purification grade monitored by reducing SDS-PAGE: extract (lane 1), total DEAE fraction eluted with 500 mM NaCl (lane 2), the affinity-purified sample (lane 3), and MW marker (M). b) Reducing SDS-PAGE (dissociating the disulfide bonds) of affinity purified samples obtained from DEAE fractions eluted with 200 mM NaCl (lane 1) and 400 mM NaCl (lane 2), MW marker (M). c) Nonreducing SDS-PAGE (keeping the disulfide bonds intact) of affinity purified samples obtained from DEAE fractions eluted with 200 mM NaCl (lane 1) and 400 mM NaCl (lane 2), MW marker (M).
