FASEB J.
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Figure 2


Figure 2. HSP27 increases p27Kip1 ubiquitination and degradation. A) HSP27 increases p27Kip1 mono-ubiquitination. Nickel pull-down experiments were performed in Cos cells transfected with Ubiquitin-His, together with p27Kip1 or HSP27 or both, in the presence of MG132 (30 µM). B) HSP27 favors degradation of ubiquitinated p27Kip1. Nickel pull-down experiments were performed as in A but in the absence of MG132.





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