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Figure 2. Spontaneous exocytosis/endocytosis of synaptic-like vesicles in cultured podocytes. A, B) The same field is taken after 2 h incubation with the antisynaptotagmin-1 lumenal Ab (Rabbit antisyt 1, Alexa Fluor 488 goat anti-rabbit IgG) (A) and after applying the Ab against the cytosolic portion of synaptotagmin 1 (mouse anti p65, Alexa Fluor 486 goat antimouse IgG) (B). Note the precise merging of fluorescent puncta in the podocyte process (C). D) The same image, taken in bright-field mode, shows the podocyte process detail. E) A podocyte process looks completely negative for the antisynaptotagmin-1 lumenal Ab after 24 h incubation with TeNT. (Rabbit antisyt 1, Alexa Fluor 488 goat anti-rabbit IgG). F) The same process looks stained by the Ab directed against the cytosolic portion of synaptotagmin 1 (mouse anti p65, Alexa Fluor 486 goat antimouse IgG). G) Merge image of E and F. H) The same image, taken in bright-field mode, shows the podocyte process detail. ctr = control medium; TeNT = medium containing the complete tetanus toxin. Scale bar = 10 µm.
