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Figure 1. EM and immunogold labeling. A) EM study of a kidney section, cut parallel to the glomerular basement membrane. In a foot process, a number of coated vesicles are located in the proximity of the slit diaphragm (SD). B) EM study of primary podocyte cell cultures. The edge of a podocyte process takes close contacts with neighboring cell processes and contains several coated vesicles. C) Immunogold EM study of a kidney section, cut perpendicular to the glomerular basement membrane. Immunogold particles are present in podocyte foot processes, showing a clear relationship or fully contained (arrow) in vesicular structures (mouse antiglutamate, 10-nm gold-conjugated goat antimouse). Scale bars (A, B) = 500 nm. Scale bar (C) = 800 nm
