Click on image to view larger version.

Figure 1. Immunohistochemical localization of the GFP in mouse ileum, liver and spleen. AC) Sections of the ileum of the mice fed with CTB-GFP expressing plant leaf. Arrows indicate presence of GFP in the intestinal epithelium as well as cells of the crypts. D) Shows a section of the ileum of a mouse fed with wild-type (untransformed) plant leaf materials. E) GFP- immunoreactivity in hepatocytes (arrows) in a mouse fed orally with CTB-GFP expressing plant. F) Section of the liver from a mouse fed with wild-type (untransformed) plant. G) Section of the liver from a mouse fed with IFN-GFP expressing plant. H) GFP-immunoreactivity in the spleen of mouse fed orally with CTB-GFP expressing plant. Arrows indicate various cells with a higher GFP content. I) Section of the spleen from a mouse fed with wild-type (untransformed) plant. J) Section of the spleen from a mouse fed with IFN-GFP expressing plant. Scale bar for AD = 50 µm. Scale bar for EJ = 25 µm.