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Figure 2. Human HSC-derived cardiomyocytes in NOD/SCID/IL2r
null recipient mice. Double FISH analysis was performed on recipient cardiac specimens using human (red) and mouse (green) centromere probes. Nuclei of human- and mouse cells were stained with 4',6'-diam idino-2-phenylidole (DAPI). Left) Cardiac tissue of the recipient mice was constituted of human chromosome– murine chromosome+ myocytes (upper, labeled as M) and human chromosome+ murine chromosome+ myocytes (upper, marked as F). Cardiomyocytes express connexin 43 peripherally (white dots). Center and right) Serial confocal images obtained from different depths of the sample confirmed that both human- and murine chromosomes were inside the same nucleus.
