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Figure 1. GFP+ ScaI+ cell-derived cardiomyocytes in CFP+ recipients. A) GFP+ Lin– ScaI+ BM cells were transplanted into neonatal CFP transgenic mice. Lambda image acquisition and linear unmixing analysis were employed to specifically detect donor-derived GFP and host-derived CFP of the samples. Control reference spectra were obtained from cardiomyocytes of host CFP transgenic mice and from BM cells of donor GFP transgenic mice. Total fluorescent light emitted by the samples was collected from 450 nM to 600 nM, and contribution of GFP and CFP was calculated based on the equation [S(
)SUM =Intensity xS()CFP+IntensityxS()GFP].If the cell is a product of cell fusion, fluorescence light emitted by the sample is constituted by GFP and CFP. If the cell differentiated from transplanted hematopoietic progenitor cells, the emitted fluorescence light of the cell is equivalent to that of GFP. B) The GFP+ myocytes show the emission spectra, which are composed of both CFP and GFP. The fluorescence intensity in different wavelengths between 450 and 600 nM is shown. The cardiomyocyte in center possesses both CFP and GFP in the cytoplasm. All the GFP+ hematopoietic cells did not express host-derived CFP.
