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Figure 2. Protein disulfide reductase (PDR) activity of the endoplasmic reticulum. For protein disulfide reductase (PDR) activity measurements 75 µg microsomal proteins were added to 1 µM di-FiTZ insulin solution in 5 mM Tris·HCl buffer in the presence of a 5:1 GSH/GSSG redox buffer (final GSH concentration 2 mM), pH 7.2 and the enhancement of fluorescence was measured as described in the Materials and Methods. Open diamonds or filled circles represent microsomal samples from control or PiZ transgenic mice, respectively. Dashed line represents the positive control in the presence of 5 mM DTT; the dotted line represents the negative control with buffer only. All data are expressed as a percentage of fluorescence reached by thioredoxin (Trx). Means ± SD of 8 independent experiments with 6 mice per data set are presented. *Significant change compared with control, P < 0.05. **Significant change compared to control, P < 0.01.
